Dr Nady Braidy
Dr Braidy is an Australian Research Council Discovery Early Career Research
Associate (2017-2020). He has previously been awarded the NHMRC Early
Career Research Fellowship (2013-2016), and the Alzheimer’s Australia
Viertel Postdoctoral Research Fellowship (2012) at The University of New
South Wales, Sydney.
Since his PhD was awarded (November 2011) he has over 97 peer reviewed
publications (H index = 21), 6 book chapters, 2 books and 1 international
patent, and he has presented orally at over 10 national and international
conferences. Recently, he was one of 5 people to be awarded the Australian
Academy of Science, Science and Industry Endowment Fund to represent
Australia at the 64th Lindau Nobel Laureate Meeting in (29th June – 4th July
2014).
In 2012, he was awarded the International Investigator of the Year Award in
Geriatric Psychoneuro-pharmacology for contribution to ageing and
neurodegenerative diseases, and received the UNSW Faculty of Medicine
Dean’s Rising Star Award for Excellence in Postdoctoral Research. In
addition, he received the Doerenkamp-Zbinden Prize in Pharmacology for the
best performance in the UNSW pharmacology major.
His drive and technical skills have enabled him to perform sophisticated
experimental work, and this has consistently produced publishable results
throughout his PhD research career. In 2009, he received the Faculty of
Medicine Dean's list award, which acknowledges his achievements as a
young researcher. He also completed a Masters in Pharmacy whilst
simultaneously publishing 7 first author papers during his PhD, and he has
also completed a Diploma in Innovation Management, and was successfully
awarded the Graduate Certificate in Research Management under the
Commercialisation Training Scheme. These were the first experiences which
linked both an academic, and industrial-based research career. Since
completing his Honours Degree in Pharmacology and Physiology in 2007
(UNSW), his research has focused on sirtuins, a family of seven mammalian
enzymes that controls cellular energetic and protects against numerous age-
related diseases.
He has also studied small molecules which indirectly activate SIRT1 and
recently identified a common mechanism by which all these molecules
allosterically activate an NAD+-synthetic enzyme leading to increased NAD+
levels and higher SIRT1 activity. This work is the subject of an international
patent which he is a co-inventor (No. PCT/AU2009/000255). In his honours
project, he successfully discovered a novel small molecule activator of NAD+
synthesis and was the first to identify such a mechanism which has wide
ranging therapeutic potential. This discovery is currently the subject of a
patent application. His strong foundation in the laboratory has been pivotal in
inspiring him to continue to be a high achiever in scientific research
Approximately 80% of his time is spent on research. The remaining 20% is
spent on teaching and student supervision. In recognition of his sound
expertise, he has been asked to co-supervise several honours and master
students during his professional career. In his immediate research
environment, he has chaired PhD student seminars, and coordinated
afternoon teas for students to foster collaboration and develop a positive
research culture. These initiatives and achievements demonstrate that he not
only has the skills to perform at the highest level as a researcher, but also
have the professional attributes and leadership skills to allow him to become a
valuable member of the Australian and international scientific community.
2017 Lecture (1) - NAD+ Precursors as Therapeutic Agents for Age-related
Degenerative Diseases
Significance: Nicotinamide adenine dinucleotide (NAD+) is an essential pyridine nucleotide
that serves as an essential cofactor and substrate for a number of critical cellular processes
involved in oxidative phosphorylation and ATP production, DNA repair, epigenetically
modulated gene expression, intracellular calcium signalling and immunological functions.
NAD+ depletion may occur in response to either excessive DNA damage due to free radical
or UV attack, resulting in significant PARP activation and a high turnover and subsequent
depletion of NAD+, and/or chronic immune activation and inflammatory cytokine production
resulting in accelerated CD38 activity and decline in NAD+ levels. Recent studies have
shown that enhancing NAD+ levels can profoundly reduce oxidative cell damage in catabolic
tissue, including the brain. Therefore, promotion of intracellular NAD+ anabolism represents
a promising therapeutic strategy for age-associated degenerative diseases in general, and is
essential to the effective realisation of multiple benefits of healthy sirtuin activity. Recent
Advances: In this presentation, we will describe and discuss recent insights regarding the
efficacy and benefits of the NAD+ precursors, nicotinamide (NAM), nicotinic acid (NA),
nicotinamide riboside (NR), and nicotinamide mononucleotide (NMN), in attenuating NAD+
decline in degenerative disease states and physiological ageing. Critical Issues: Results
obtained in recent years have shown that NAD+ precursors can play important protective
roles in several diseases. However, in some cases, these precursors may have varying
differences in their ability to enhance NAD+ synthesis via their location in the NAD+
anabolic pathway. Moreover, formulation of these precursors represents an additional issue
for consideration due to their poor stability and highly reactive nature. As well, increased
synthesis of NAD+ promote protective cell responses, further demonstrating that NAD+ is a
regulatory molecule associated with several biochemical pathways. Future Directions: In the
next few years, the refinement of personalised therapy for the use of NAD+ precursors,
allowing the administration of specific NAD+ precursors on the context of patients NAD+
levels will lead to a better understanding of the therapeutic role of NAD+ precursors in
human diseases and ageing.
2017 Lecture (2) - Promotion of Cellular NAD+ Anabolism as a Strategy to Improve Cellular Senescence
Significance: Adequate cellular nicotinamide adenine dinucleotide (NAD+) levels
promote DNA repair, activate sirtuin-linked deacetylation of histones and p53,
promoting cellular metabolism and continuing viability. Recent Advances: In this
presentation, We examined the effect of aging on intracellular NAD+ metabolism
in different brain regions from young (3 months), middle aged (12 months) and
older adults (24 months) female Wistar rats. Lipid peroxidation was measured
using an antibody to 4-hydroxynonenal. Protein oxidation was measured using gas
chromatography mass spectrometry. DNA damage, PARP and CD38 activities,
and intracellular NAD and NADH levels were measured using well established
colorimetric assays. Critical Issues: Our results are the first to show a significant
decline in intracellular NAD+ levels and NAD:NADH ratio with ageing in the
CNS, occurring in parallel to an increase in lipid peroxidation and protein
oxidation (o- and m- tyrosine) and a decline in total antioxidant capacity.
Hyperphosphorylation of H2AX levels was also observed together with increased
PARP-1 expression, and CD38 activity, concomitantly with reduced NAD+ and
ATP levels and SIRT1 function in the cortex, brainstem, hippocampus and
cerebellum. Reduced activity of mitochondrial complex I-IV and impaired
maximum mitochondrial respiration rate were also observed in the ageing rat brain.
Among the multiple physiological pathways associated with NAD+ catabolism,
our discovery of CD38 as the major regulator of cellular NAD+ levels in rat
neurons indicates that CD38 is a promising therapeutic target for the treatment of
age-related neurodegenerative diseases. Future Directions: Chronic oxidative
stress can disrupt several physiological processes and lead to a decrease in the
intracellular pool of NAD+ leading to impaired function of NAD+-dependent
pathways including oxidative phosphorylation, and telomere attrition. Promotion
of cellular NAD+ anabolism may therefore prove a promising pharmacological
strategy to delay the onset of senescence in ageing and slow cognitive decline.